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991.
T Oniki Y Hashimoto S Shimizu T Kakuta M Yajima F Numano 《Heart (British Cardiac Society)》1992,68(4):425-429
Objective—To investigate the effects of heart rate on the Doppler measurements of left ventricular function and to determine the normal pattern of rate dependency.Setting—University hospital specialising in internal medicine.Participants—14 healthy male volunteers 10 of whom were studied.Intervention: Transoesophageal atrial pacing.Main outcome measures—At paced rates of 70, 80, and 90 ppm the ratio of early to late peak transmitral flow velocity (E/A) was 1·97 (0·28), 1·49 (0·21), and 0·95 (0·11) respectively; the ratio of early to late time-velocity integrals of transmitral flow (Ei/Ai) was 3·03 (0·51), 2·11 (0·24), and 1·14 (0·30) respectively; and the atrial filling fraction (AFF) was 0·17 (0·03), 0·21, (0·04), and 0·24 (0·04) (mean (SD)).Results—Heart rate showed a linear correlation with E/A (r2 = 0·806), Ei/Ai (r2 = 0·838), and AFF (r2 = 0·343). Neither the peak aortic flow velocity or the mean aortic flow acceleration showed significant changes during pacing at rates of 70, 80, 90, and 100 ppm.Conclusions—E/A and Ei/Ai can be expected to decrease by 0·5 and 0·9 for each increase of 10 beats/min in heart rate.Knowledge of this relation may be useful for the development of algorithms to correct for heart rate when diastolic function is assessed. 相似文献
992.
Tomohiro Miyai Shintaro Hojyo Tomokatsu Ikawa Masami Kawamura Tarou Irié Hideki Ogura Atsushi Hijikata Bum-Ho Bin Takuwa Yasuda Hiroshi Kitamura Manabu Nakayama Osamu Ohara Hisahiro Yoshida Haruhiko Koseki Kenji Mishima Toshiyuki Fukada 《Proceedings of the National Academy of Sciences of the United States of America》2014,111(32):11780-11785
The immune system is influenced by the vital zinc (Zn) status, and Zn deficiency triggers lymphopenia; however, the mechanisms underlying Zn-mediated lymphocyte maintenance remain elusive. Here we investigated ZIP10, a Zn transporter expressed in the early B-cell developmental process. Genetic ablation of Zip10 in early B-cell stages resulted in significant reductions in B-cell populations, and the inducible deletion of Zip10 in pro-B cells increased the caspase activity in parallel with a decrease in intracellular Zn levels. Similarly, the depletion of intracellular Zn by a chemical chelator resulted in spontaneous caspase activation leading to cell death. Collectively, these findings indicated that ZIP10-mediated Zn homeostasis is essential for early B-cell survival. Moreover, we found that ZIP10 expression was regulated by JAK-STAT pathways, and its expression was correlated with STAT activation in human B-cell lymphoma, indicating that the JAK-STAT-ZIP10-Zn signaling axis influences the B-cell homeostasis. Our results establish a role of ZIP10 in cell survival during early B-cell development, and underscore the importance of Zn homeostasis in immune system maintenance.Zinc (Zn) has wide-ranging effects on immunity. Zn deficiency has uncovered the importance of Zn homeostasis in immune cell maintenance and function (1). Dramatic effects of Zn on immunity have been observed in several immune and allergy-related cells, including lymphocytes such as B cells (2–6). B cells develop in the bone marrow (BM); the initial commitment to pro-B cells is followed by their differentiation into pre-B cells, and subsequently into immature B cells, which express the B-cell receptor on their surface (7). The immature B cells reach the spleen as transitional B cells, further differentiating into follicular or marginal zone mature B cells (7). Although the perturbation of Zn homeostasis causes splenic atrophy associated with lymphocyte reduction, and compromises cellular and humoral immune responses (6), the mechanisms underlying how Zn controls immune cell function, and in particular, the impact on early B-cell development, have been largely unknown.Zn homeostasis is tightly controlled by Zn transporter family members, Zrt- and Irt-like proteins (ZIPs, Zn importers) and zinc transporters (ZnTs, Zn exporters) (8), and recent studies revealed that alterations in Zn homeostasis mediated by specific Zn transporters play indispensable roles in a variety of cellular events (9). The intestinal Zn transporter ZIP4 is important for the initial absorption of dietary Zn, and patients with mutations in the SLC39A4/ZIP4 gene suffer from the inherited disorder acrodermatitis enteropathica (10, 11). ZIP13 controls the formation of bone, teeth, and connective tissues by modulating BMP/TGF-β signaling (12), and its loss-of-function mutation causes spondylocheiro dysplastic Ehlers-Danlos syndrome in humans (12, 13). ZIP14 controls systemic growth by regulating G protein-coupled receptor (GPCR) signaling (14), and ZIP8 is involved in osteoarthritis (15) and negatively manipulates NF-κB activation (16). In addition, ZnT5 regulates cytokine production by controlling the activation of protein kinase C upon antigen exposure in mast cells (17). Thus, Zn homeostasis mediated by Zn transporters is linked to a wide variety of biological and regulatory functions, and the disruption of a Zn transporter-Zn axis can lead to various symptoms in the absence of redundant machinery (18).Here we demonstrate a definitive role of ZIP10 in early B-cell development. We found that a loss of ZIP10 during an early B-cell stage specifically abrogated cell survival, resulting in the absence of mature B cells, which led to splenoatrophy and reduced Ig levels. The inducible deletion of Zip10 in pro-B cells increased the caspase activity because of the reduced intracellular Zn level, leading to cell death. This phenomenon was mimicked by the intracellular chelation of Zn. These findings indicated that Zn homeostasis via ZIP10 plays an indispensable role in early B-cell survival. We also demonstrated that the ZIP10 expression levels were regulated by STAT3/STAT5 activation, and that ZIP10 was highly expressed in human B-cell lymphoma samples in which both STAT proteins were activated, indicating that the JAK-STAT-ZIP10-Zn signaling axis is important for B-cell maintenance. Our results establish a functional link between ZIP10 and the survival of early stages of B cells, revealing a molecular mechanism underlying the requirement of Zn for maintenance of the immune system. 相似文献
993.
Yamamori T White AR Mattagajasingh I Khanday FA Haile A Qi B Jeon BH Bugayenko A Kasuno K Berkowitz DE Irani K 《Journal of molecular and cellular cardiology》2005,39(6):101-995
The p66shc adaptor protein mediates age-associated oxidative stress. We examined the role of p66shc in endothelial nitric oxide synthase (eNOS) signaling. Overexpression of p66shc inhibited eNOS-dependent NO production. RNAi-mediated down-regulation of endogenous p66shc led to activation of the proto-oncogene ras, and Akt kinase, with a corresponding increase in phosphorylation of eNOS at S1177 (S1179 on bovine eNOS). In rat aortic rings, down-regulation of p66shc suppressed the vasoconstrictor response to phenyephrine that was abrogated by treatment with the NOS inhibitor l-NAME, and enhanced vasodilation induced by sub-maximal doses of acetylcholine. These findings highlight a pivotal role for p66shc in inhibiting endothelial NO production, and endothelium-dependent vasorelaxation, that may provide important mechanistic information about endothelial dysfunction seen with aging. 相似文献
994.
Ca2+ activity at GABAB receptors constitutively promotes metabotropic glutamate signaling in the absence of GABA 下载免费PDF全文
Tabata T Araishi K Hashimoto K Hashimotodani Y van der Putten H Bettler B Kano M 《Proceedings of the National Academy of Sciences of the United States of America》2004,101(48):16952-16957
Type B gamma-aminobutyric acid receptor (GABABR) is a G protein-coupled receptor that regulates neurotransmitter release and neuronal excitability throughout the brain. In various neurons, GABABRs are concentrated at excitatory synapses. Although these receptors are assumed to respond to GABA spillover from neighboring inhibitory synapses, their function is not fully understood. Here we show a previously undescribed function of GABABR exerted independent of GABA. In cerebellar Purkinje cells, interaction of GABABR with extracellular Ca2+ (Ca(2+)o) leads to a constitutive increase in the glutamate sensitivity of metabotropic glutamate receptor 1 (mGluR1). mGluR1 sensitization is clearly mediated by GABABR because it is absent in GABABR1 subunit-knockout cells. However, the mGluR1 sensitization does not require G(i/o) proteins that mediate the GABABR's classical functions. Moreover, coimmunoprecipitation reveals complex formation between GABABR and mGluR1 in the cerebellum. These findings demonstrate that GABABR can act as Ca(2+)o-dependent cofactors to enhance neuronal metabotropic glutamate signaling. 相似文献
995.
996.
Vichuda Sattayarut Chalathorn Chanthad Pongtanawat Khemthong Sanchai Kuboon Thanthamrong Wanchaem Mayuree Phonyiem Michiko Obata Masatsugu Fujishige Kenji Takeuchi Winadda Wongwiriyapan Paisan Khanchaitit Morinobu Endo 《RSC advances》2019,9(18):9878
In this study, nitrogen-enriched activated carbon from silkworm pupae waste (P-AC) was successfully prepared and its electrochemical performances in aqueous and organic electrolytes were investigated. Silkworm pupae waste is beneficial because it is a nitrogen-enriched, inexpensive, and locally available material. The preparation process includes hydrothermal treatment of the silkworm pupae waste at 200 °C, and chemical activation using zinc chloride at activation temperatures of 700, 800 and 900 °C (P700, P800, and P900, respectively). The nitrogen content in the P-ACs was approximately 3.8–6.4 at%, decreasing with activation temperature, while the surface area was approximately 1062–1267 m2 g−1, increasing with activation temperature. Compared to a commercial AC, the P-ACs show higher nitrogen content but lower surface area. Furthermore, the P800 exhibited superior specific capacitance (154.6 and 91.6 F g−1 in aqueous and organic electrolytes) compared to a commercial AC despite possessing smaller surface area. The high nitrogen content enhanced the pseudocapacitance and improved the electrical conductivity of the P-ACs. These properties were confirmed by relatively low series and charge transfer resistances, a capacity retention higher than 88% at a current density of 0.5 A g−1 and excellent cycling stability demonstrated by maintaining 97.6% of its capacitance after 3000 cycles. These results demonstrate that silkworm pupae waste is a viable source of nitrogen-enriched AC for application in supercapacitors.Nitrogen-enriched activated carbon from silkworm pupae prepared by hydrothermal treatment and chemical activation exhibited superior specific capacitance with excellent cycling stability. 相似文献
997.
Daisuke Hirose Samuel Budi Wardhana Kusuma Shuhei Nomura Makoto Yamaguchi Yoshiro Yasaka Ryohei Kakuchi Kenji Takahashi 《RSC advances》2019,9(7):4048
The role of 1-ethyl-3-methylimidazolium (Emim) carboxylate-type ionic liquid (IL) as the solvent and organocatalyst for transesterification reaction of cellulose was investigated. The reported method using Emim acetate and vinyl ester caused an undesired side reaction: the acetate anion derived from EmimOAc was introduced into cellulose ester. To improve the reaction system, ILs with a high cellulose solubility, a high degree of substitution (DS) value, and low side-reaction were systematically explored. Newly synthesized Emim p-anisate and a mixed solvent system achieved the transesterification reaction of cellulose with a high DS value derived from the employed vinyl esters (DS > 2.9), and a low DS value derived from side reaction (selectivity > 99%).The role of 1-ethyl-3-methylimidazolium carboxylate-type ionic liquid as the solvent and organocatalyst for transesterification reaction of cellulose was investigated. 相似文献
998.
999.
1000.
Yasuko Okumura Noriyuki Sugiyama Susumu Tanimura Masashi Nishida Kenji Hamaoka Michiaki Kohno Takahiko Yokoyama 《ACTA HISTOCHEMICA ET CYTOCHEMICA》2009,42(2):39-45
Nephronophthisis (NPHP) is the most frequent genetic cause of end-stage kidney disease in children and young adults. Inv mice are a model for human nephronophthisis type 2 (NPHP2) and characterized by multiple renal cysts and situs inversus. Renal epithelial cells in inv cystic kidneys show increased cell proliferation. We studied the ERK pathway to understand the mechanisms that induce cell proliferation and renal cyst progression in inv kidneys. We studied the effects of ERK suppression by administering PD184352, an oral mitogen-activated protein kinase kinase (MEK) inhibitor on renal cyst expansion, extracellular signal-regulated protein kinase (ERK) activity, bromo-deoxyuridine (BrdU) incorporation and expression of cell-cycle regulators in invΔC kidneys. Phosphorylated ERK (p-ERK) level increased along with renal cyst enlargement. Cell-cycle regulators showed a high level of expression in invΔC kidneys. PD184352 successfully decreased p-ERK level and inhibited renal cyst enlargement. The inhibitor also decreased expression of cell-cycle regulators and BrdU incorporation in renal epithelial cells. The present results showed that ERK regulated renal cell proliferation and cyst expansion in inv mutants. 相似文献